TY - JOUR A1 - Kochiwa, Hiromi A1 - Suzuki, Ryosuke A1 - Washio, Takanori A1 - Saito, Rintaro A1 - Phase, The RIKEN Genome Exploration Research Group A1 - Bono, Hidemasa A1 - Carninci, Piero A1 - Okazaki, Yasushi A1 - Miki, Rika A1 - Hayashizaki, Yoshihide A1 - Tomita, Masaru T1 - Inferring Alternative Splicing Patterns in Mouse from a Full-Length cDNA Library and Microarray Data Y1 - 2002/08/01 JF - Genome Research JO - Genome Research SP - 1286 EP - 1293 DO - 10.1101/gr.220302 VL - 12 IS - 8 UR - http://genome.cshlp.org/content/12/8/1286.abstract N2 - Although many studies on alternative splicing of specific genes have been reported in the literature, the general mechanism that regulates alternative splicing has not been clearly understood. In this study, we systematically aligned each pair of the 21,076 cDNA sequences ofMus musculus, searched for putative alternative splicing patterns, and constructed a list of potential alternative splicing sites. Two cDNAs are suspected to be alternatively spliced and originating from a common gene if they share most of their region with a high degree of sequence homology, but parts of the sequences are very distinctive or deleted in either cDNA. The list contains the following information: (1) tissue, (2) developmental stage, (3) sequences around splice sites, (4) the length of each gapped region, and (5) other comments. The list is available athttp://www.bioinfo.sfc.keio.ac.jp/intron. Our results have predicted a number of unreported alternatively spliced genes, some of which are expressed only in a specific tissue or at a specific developmental stage. ER -