RT Journal
A1 Rappsilber, Juri
A1 Ryder, Ursula
A1 Lamond, Angus I.
A1 Mann, Matthias
T1 Large-Scale Proteomic Analysis of the Human Spliceosome
JF Genome Research 
JO Genome Research 
YR 2002 
FD August 01 
VO 12 
IS 8 
SP 1231 
OP 1245 
DO 10.1101/gr.473902 
UL http://genome.cshlp.org/content/12/8/1231.abstract 
AB In a previous proteomic study of the human spliceosome, we identified 42 spliceosome-associated factors, including 19 novel ones. Using enhanced mass spectrometric tools and improved databases, we now report identification of 311 proteins that copurify with splicing complexes assembled on two separate pre-mRNAs. All known essential human splicing factors were found, and 96 novel proteins were identified, of which 55 contain domains directly linking them to functions in splicing/RNA processing. We also detected 20 proteins related to transcription, which indicates a direct connection between this process and splicing. This investigation provides the most detailed inventory of human spliceosome-associated factors to date, and the data indicate a number of interesting links coordinating splicing with other steps in the gene expression pathway.