RT Journal
A1 Oshiro, Guy
A1 Wodicka, Lisa M.
A1 Washburn, Michael P.
A1 Yates, John R.
A1 Lockhart, David J.
A1 Winzeler, Elizabeth A.
T1 Parallel Identification of New Genes in Saccharomyces cerevisiae

JF Genome Research 
JO Genome Research 
YR 2002 
FD August 01 
VO 12 
IS 8 
SP 1210 
OP 1220 
DO 10.1101/gr.226802 
UL http://genome.cshlp.org/content/12/8/1210.abstract 
AB Short open reading frames (ORFs) occur frequently in primary genome sequence. Distinguishing bona fide small genes from the tens of thousands of short ORFs is one of the most challenging aspects of genome annotation. Direct experimental evidence is often required. Here we use a combination of expression profiling and mass spectrometry to verify the independent transcription of 138 and the translation of 50 previously nonannotated genes in the Saccharomyces cerevisiae genome. Through combined evidence, we propose the addition of 62 new genes to the genome and provide experimental support for the inclusion of 10 previously identified genes.[The following individuals kindly provided reagents, samples, or unpublished information as indicated in the paper: V. Velculescu. Supplementary material is available online at http://www.genome.org.]