TY  - JOUR
A1  - Simin, Karl
A1  - Scuderi, Anne
A1  - Reamey, James
A1  - Dunn, Diane
A1  - Weiss, Robert
A1  - Metherall, James E.
A1  - Letsou, Anthea
T1  - Profiling Patterned Transcripts in Drosophila Embryos
Y1  - 2002/07/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1040 
EP  - 1047 
DO  - 10.1101/gr.84402 
VL  - 12 
IS  - 7 
UR  - http://genome.cshlp.org/content/12/7/1040.abstract 
N2  - Here we describe a high-throughput screen to isolate transcripts with spatially restricted patterns of expression in early embryos. Our approach utilizes robotic automation for rapid analysis of sequence-selected cDNAs in a whole-mount in situ hybridization assay. We determined the spatial distribution of a random collection of 778 different genes from an embryonic cDNA library and show that a significant fraction of these exhibit patterned profiles of expression. In addition, gene ontology studies revealed groups of gene products exhibiting shared expression patterns, providing new insights into the largely overlooked effector molecules that function in development. As described in this paper, automated hybridization to whole-mount embryos in situ proved to be straightforward and provided us with a very powerful method for the global survey of gene expression in early embryos. From the perspective of biological significance, our finding that many spatially restricted transcripts correspond to loci encoding novel transcripts that have not been previously identified in nearly saturating genetic screens for maternal effect and zygotic lethals is particularly notable.[Supplementary material available online at http://www.genome.org. The following individuals kindly provided reagents, samples, or unpublished information as indicated in the paper: N. Brown] 
ER  -