TY - JOUR A1 - Wang, Jun A1 - Wong, Gane Ka-Shu A1 - Ni, Peixiang A1 - Han, Yujun A1 - Huang, Xiangang A1 - Zhang, Jianguo A1 - Ye, Chen A1 - Zhang, Yong A1 - Hu, Jianfei A1 - Zhang, Kunlin A1 - Xu, Xin A1 - Cong, Lijuan A1 - Lu, Hong A1 - Ren, Xide A1 - Ren, Xiaoyu A1 - He, Jun A1 - Tao, Lin A1 - Passey, Douglas A. A1 - Wang, Jian A1 - Yang, Huanming A1 - Yu, Jun A1 - Li, Songgang T1 - RePS: A Sequence Assembler That Masks Exact Repeats Identified from the Shotgun Data Y1 - 2002/05/01 JF - Genome Research JO - Genome Research SP - 824 EP - 831 DO - 10.1101/gr.165102 VL - 12 IS - 5 UR - http://genome.cshlp.org/content/12/5/824.abstract N2 - We describe a sequence assembler, RePS(repeat-masked Phrap with scaffolding), that explicitly identifies exact 20mer repeats from the shotgun data and removes them prior to the assembly. The established software Phrap is used to compute meaningful error probabilities for each base. Clone-end-pairing information is used to construct scaffolds that order and orient the contigs. We show with real data for human and rice that reasonable assemblies are possible even at coverages of only 4× to 6×, despite having up to 42.2% in exact repeats.[The following individuals kindly provided reagents, samples, or unpublished information as indicated in the paper: P. Green and A.F. Smit.] ER -