RT Journal
A1 Zhao, Qiang
A1 Zhang, Yu
A1 Cheng, Zhukuan
A1 Chen, Mingsheng
A1 Wang, Shengyue
A1 Feng, Qi
A1 Huang, Yucheng
A1 Li, Ying
A1 Tang, Yesheng
A1 Zhou, Bo
A1 Chen, Zhehua
A1 Yu, Shuliang
A1 Zhu, Jingjie
A1 Hu, Xin
A1 Mu, Jie
A1 Ying, Kai
A1 Hao, Pei
A1 Zhang, Lei
A1 Lu, Yiqi
A1 Zhang, Lei S.
A1 Liu, Yilei
A1 Yu, Zhen
A1 Fan, Danlin
A1 Weng, Qijun
A1 Chen, Ling
A1 Lu, Tingting
A1 Liu, Xiaohui
A1 Jia, Peixin
A1 Sun, Tongguo
A1 Wu, Yongrui
A1 Zhang, Yujun
A1 Lu, Ying
A1 Li, Can
A1 Wang, Rong
A1 Lei, Haiyan
A1 Li, Tao
A1 Hu, Hao
A1 Wu, Mei
A1 Zhang, Runquan
A1 Guan, Jianping
A1 Zhu, Jia
A1 Fu, Gang
A1 Gu, Minghong
A1 Hong, Guofan
A1 Xue, Yongbiao
A1 Wing, Rod
A1 Jiang, Jiming
A1 Han, Bin
T1 A Fine Physical Map of the Rice Chromosome 4
JF Genome Research 
JO Genome Research 
YR 2002 
FD May 01 
VO 12 
IS 5 
SP 817 
OP 823 
DO 10.1101/gr.48902 
UL http://genome.cshlp.org/content/12/5/817.abstract 
AB As part of an international effort to completely sequence the rice genome, we have produced a fine bacterial artificial chromosome (BAC)-based physical map of the Oryza sativa japonicaNipponbare chromosome 4 through an integration of 114 sequenced BAC clones from a taxonomically related subspecies O. sativa indica Guangluai 4 and 182 RFLP and 407 expressed sequence tag (EST) markers with the fingerprinted data of the Nipponbare genome. The map consists of 11 contigs with a total length of 34.5 Mb covering 94% of the estimated chromosome size (36.8 Mb). BAC clones corresponding to telomeres, as well as to the centromere position, were determined by BAC-pachytene chromosome fluorescence in situ hybridization (FISH). This gave rise to an estimated length ratio of 5.13 for the long arm and 2.9 for the short arm (on the basis of the physical map), which indicates that the short arm is a highly condensed one. The FISH analysis and physical mapping also showed that the short arm and the pericentromeric region of the long arm are rich in heterochromatin, which occupied 45% of the chromosome, indicating that this chromosome is likely very difficult to sequence. To our knowledge, this map provides the first example of a rapid and reliable physical mapping on the basis of the integration of the data from two taxonomically related subspecies.[The following individuals and institutions kindly provided reagents, samples, or unpublished information as indicated in the paper: S. McCouch, T. Sasaki, and Monsanto.]