TY  - JOUR
A1  - Zhao, Qiang
A1  - Zhang, Yu
A1  - Cheng, Zhukuan
A1  - Chen, Mingsheng
A1  - Wang, Shengyue
A1  - Feng, Qi
A1  - Huang, Yucheng
A1  - Li, Ying
A1  - Tang, Yesheng
A1  - Zhou, Bo
A1  - Chen, Zhehua
A1  - Yu, Shuliang
A1  - Zhu, Jingjie
A1  - Hu, Xin
A1  - Mu, Jie
A1  - Ying, Kai
A1  - Hao, Pei
A1  - Zhang, Lei
A1  - Lu, Yiqi
A1  - Zhang, Lei S.
A1  - Liu, Yilei
A1  - Yu, Zhen
A1  - Fan, Danlin
A1  - Weng, Qijun
A1  - Chen, Ling
A1  - Lu, Tingting
A1  - Liu, Xiaohui
A1  - Jia, Peixin
A1  - Sun, Tongguo
A1  - Wu, Yongrui
A1  - Zhang, Yujun
A1  - Lu, Ying
A1  - Li, Can
A1  - Wang, Rong
A1  - Lei, Haiyan
A1  - Li, Tao
A1  - Hu, Hao
A1  - Wu, Mei
A1  - Zhang, Runquan
A1  - Guan, Jianping
A1  - Zhu, Jia
A1  - Fu, Gang
A1  - Gu, Minghong
A1  - Hong, Guofan
A1  - Xue, Yongbiao
A1  - Wing, Rod
A1  - Jiang, Jiming
A1  - Han, Bin
T1  - A Fine Physical Map of the Rice Chromosome 4
Y1  - 2002/05/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 817 
EP  - 823 
DO  - 10.1101/gr.48902 
VL  - 12 
IS  - 5 
UR  - http://genome.cshlp.org/content/12/5/817.abstract 
N2  - As part of an international effort to completely sequence the rice genome, we have produced a fine bacterial artificial chromosome (BAC)-based physical map of the Oryza sativa japonicaNipponbare chromosome 4 through an integration of 114 sequenced BAC clones from a taxonomically related subspecies O. sativa indica Guangluai 4 and 182 RFLP and 407 expressed sequence tag (EST) markers with the fingerprinted data of the Nipponbare genome. The map consists of 11 contigs with a total length of 34.5 Mb covering 94% of the estimated chromosome size (36.8 Mb). BAC clones corresponding to telomeres, as well as to the centromere position, were determined by BAC-pachytene chromosome fluorescence in situ hybridization (FISH). This gave rise to an estimated length ratio of 5.13 for the long arm and 2.9 for the short arm (on the basis of the physical map), which indicates that the short arm is a highly condensed one. The FISH analysis and physical mapping also showed that the short arm and the pericentromeric region of the long arm are rich in heterochromatin, which occupied 45% of the chromosome, indicating that this chromosome is likely very difficult to sequence. To our knowledge, this map provides the first example of a rapid and reliable physical mapping on the basis of the integration of the data from two taxonomically related subspecies.[The following individuals and institutions kindly provided reagents, samples, or unpublished information as indicated in the paper: S. McCouch, T. Sasaki, and Monsanto.] 
ER  -