@article{Zhao01052002,
author = {Zhao, Qiang and Zhang, Yu and Cheng, Zhukuan and Chen, Mingsheng and Wang, Shengyue and Feng, Qi and Huang, Yucheng and Li, Ying and Tang, Yesheng and Zhou, Bo and Chen, Zhehua and Yu, Shuliang and Zhu, Jingjie and Hu, Xin and Mu, Jie and Ying, Kai and Hao, Pei and Zhang, Lei and Lu, Yiqi and Zhang, Lei S. and Liu, Yilei and Yu, Zhen and Fan, Danlin and Weng, Qijun and Chen, Ling and Lu, Tingting and Liu, Xiaohui and Jia, Peixin and Sun, Tongguo and Wu, Yongrui and Zhang, Yujun and Lu, Ying and Li, Can and Wang, Rong and Lei, Haiyan and Li, Tao and Hu, Hao and Wu, Mei and Zhang, Runquan and Guan, Jianping and Zhu, Jia and Fu, Gang and Gu, Minghong and Hong, Guofan and Xue, Yongbiao and Wing, Rod and Jiang, Jiming and Han, Bin}, 
title = {A Fine Physical Map of the Rice Chromosome 4},
volume = {12}, 
number = {5}, 
pages = {817-823}, 
year = {2002}, 
doi = {10.1101/gr.48902}, 
abstract ={As part of an international effort to completely sequence the rice genome, we have produced a fine bacterial artificial chromosome (BAC)-based physical map of the Oryza sativa japonicaNipponbare chromosome 4 through an integration of 114 sequenced BAC clones from a taxonomically related subspecies O. sativa indica Guangluai 4 and 182 RFLP and 407 expressed sequence tag (EST) markers with the fingerprinted data of the Nipponbare genome. The map consists of 11 contigs with a total length of 34.5 Mb covering 94% of the estimated chromosome size (36.8 Mb). BAC clones corresponding to telomeres, as well as to the centromere position, were determined by BAC-pachytene chromosome fluorescence in situ hybridization (FISH). This gave rise to an estimated length ratio of 5.13 for the long arm and 2.9 for the short arm (on the basis of the physical map), which indicates that the short arm is a highly condensed one. The FISH analysis and physical mapping also showed that the short arm and the pericentromeric region of the long arm are rich in heterochromatin, which occupied 45% of the chromosome, indicating that this chromosome is likely very difficult to sequence. To our knowledge, this map provides the first example of a rapid and reliable physical mapping on the basis of the integration of the data from two taxonomically related subspecies.[The following individuals and institutions kindly provided reagents, samples, or unpublished information as indicated in the paper: S. McCouch, T. Sasaki, and Monsanto.]}, 
URL = {http://genome.cshlp.org/content/12/5/817.abstract}, 
eprint = {http://genome.cshlp.org/content/12/5/817.full.pdf+html}, 
journal = {Genome Research} 
}