RT Journal
A1 Bao, Qiyu
A1 Tian, Yuqing
A1 Li, Wei
A1 Xu, Zuyuan
A1 Xuan, Zhenyu
A1 Hu, Songnian
A1 Dong, Wei
A1 Yang, Jian
A1 Chen, Yanjiong
A1 Xue, Yanfen
A1 Xu, Yi
A1 Lai, Xiaoqin
A1 Huang, Li
A1 Dong, Xiuzhu
A1 Ma, Yanhe
A1 Ling, Lunjiang
A1 Tan, Huarong
A1 Chen, Runsheng
A1 Wang, Jian
A1 Yu, Jun
A1 Yang, Huanming
T1 A Complete Sequence of the T. tengcongensis Genome
JF Genome Research 
JO Genome Research 
YR 2002 
FD May 01 
VO 12 
IS 5 
SP 689 
OP 700 
DO 10.1101/gr.219302 
UL http://genome.cshlp.org/content/12/5/689.abstract 
AB 
Thermoanaerobacter tengcongensis is a rod-shaped, gram-negative, anaerobic eubacterium that was isolated from a freshwater hot spring in Tengchong, China. Using a whole-genome-shotgun method, we sequenced its 2,689,445-bp genome from an isolate, MB4T (Genbank accession no. AE008691). The genome encodes 2588 predicted coding sequences (CDS). Among them, 1764 (68.2%) are classified according to homology to other documented proteins, and the rest, 824 CDS (31.8%), are functionally unknown. One of the interesting features of the T. tengcongensis genome is that 86.7% of its genes are encoded on the leading strand of DNA replication. Based on protein sequence similarity, the T. tengcongensis genome is most similar to that of Bacillus halodurans, a mesophilic eubacterium, among all fully sequenced prokaryotic genomes up to date. Computational analysis on genes involved in basic metabolic pathways supports the experimental discovery that T. tengcongensis metabolizes sugars as principal energy and carbon source and utilizes thiosulfate and element sulfur, but not sulfate, as electron acceptors. T. tengcongensis, as a gram-negative rod by empirical definitions (such as staining), shares many genes that are characteristics of gram-positive bacteria whereas it is missing molecular components unique to gram-negative bacteria. A strong correlation between the G + C content of tDNA and rDNA genes and the optimal growth temperature is found among the sequenced thermophiles. It is concluded that thermophiles are a biologically and phylogenetically divergent group of prokaryotes that have converged to sustain extreme environmental conditions over evolutionary timescale.[Supplemental material is available online athttp://www.genome.org.]