TY  - JOUR
A1  - Doi, Nobuhide
A1  - Takashima, Hideaki
A1  - Kinjo, Masataka
A1  - Sakata, Kyoko
A1  - Kawahashi, Yuko
A1  - Oishi, Yuko
A1  - Oyama, Rieko
A1  - Miyamoto-Sato, Etsuko
A1  - Sawasaki, Tatsuya
A1  - Endo, Yaeta
A1  - Yanagawa, Hiroshi
T1  - Novel Fluorescence Labeling and High-Throughput Assay Technologies for In Vitro Analysis of Protein Interactions
Y1  - 2002/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 487 
EP  - 492 
DO  - 10.1101/gr.218802 
VL  - 12 
IS  - 3 
UR  - http://genome.cshlp.org/content/12/3/487.abstract 
N2  - We developed and tested a simple method for fluorescence labeling and interaction analysis of proteins based on a highly efficient in vitro translation system combined with high-throughput technologies such as microarrays and fluorescence cross-correlation spectroscopy (FCCS). By use of puromycin analogs linked to various fluorophores through a deoxycytidylic acid linker, a single fluorophore can be efficiently incorporated into a protein at the carboxyl terminus during in vitro translation. We confirmed that the resulting fluorescently labeled proteins are useful for probing protein–protein and protein–DNA interactions by means of pulldown assay, DNA microarrays, and FCCS in model experiments. These fluorescence assay systems can be easily extended to highly parallel analysis of protein interactions in studies of functional genomics.[Online supplementary material available at http://www.genome.org.] 
ER  -