RT Journal
A1 Xu, C. Wilson
T1 High-Density Cell Microarrays for Parallel Functional Determinations
JF Genome Research 
JO Genome Research 
YR 2002 
FD March 01 
VO 12 
IS 3 
SP 482 
OP 486 
DO 10.1101/gr.213002 
UL http://genome.cshlp.org/content/12/3/482.abstract 
AB Whole-genome sequencing projects have generated a wealth of gene sequences from a variety of organisms. A major challenge is to rapidly uncover gene regulatory circuits and their functional manifestations at the cellular level. Here we report the coupled fabrication of nanocraters ranging in size from 100 pL to 1.5 nL on permeable membranes for culturing cells. Using this approach, we developed bacterial and yeast cell microarrays that allowed phenotypic determinations of gene activities and drug targets on a large scale. Cell microarrays will therefore be a particularly useful tool for studying phenotypes of gene activities on a genome-wide scale.