RT Journal
A1 Grisart, Bernard
A1 Coppieters, Wouter
A1 Farnir, Frédéric
A1 Karim, Latifa
A1 Ford, Christine
A1 Berzi, Paulette
A1 Cambisano, Nadine
A1 Mni, Myriam
A1 Reid, Suzanne
A1 Simon, Patricia
A1 Spelman, Richard
A1 Georges, Michel
A1 Snell, Russell
T1 Positional Candidate Cloning of a QTL in Dairy Cattle: Identification of a Missense Mutation in the Bovine DGAT1 Gene with Major Effect on Milk Yield and Composition
JF Genome Research 
JO Genome Research 
YR 2002 
FD February 01 
VO 12 
IS 2 
SP 222 
OP 231 
DO 10.1101/gr.224202 
UL http://genome.cshlp.org/content/12/2/222.abstract 
AB We recently mapped a quantitative trait locus (QTL) with a major effect on milk composition—particularly fat content—to the centromeric end of bovine chromosome 14. We subsequently exploited linkage disequilibrium to refine the map position of this QTL to a 3-cM chromosome interval bounded by microsatellite markers BULGE13and BULGE09. We herein report the positional candidate cloning of this QTL, involving (1) the construction of a BAC contig spanning the corresponding marker interval, (2) the demonstration that a very strong candidate gene, acylCoA:diacylglycerol acyltransferase (DGAT1), maps to that contig, and (3) the identification of a nonconservative K232A substitution in the DGAT1 gene with a major effect on milk fat content and other milk characteristics.[The sequence data described in this paper have been submitted to the GenBank data library under accession number AY065621.]