TY  - JOUR
A1  - Grisart, Bernard
A1  - Coppieters, Wouter
A1  - Farnir, Frédéric
A1  - Karim, Latifa
A1  - Ford, Christine
A1  - Berzi, Paulette
A1  - Cambisano, Nadine
A1  - Mni, Myriam
A1  - Reid, Suzanne
A1  - Simon, Patricia
A1  - Spelman, Richard
A1  - Georges, Michel
A1  - Snell, Russell
T1  - Positional Candidate Cloning of a QTL in Dairy Cattle: Identification of a Missense Mutation in the Bovine DGAT1 Gene with Major Effect on Milk Yield and Composition
Y1  - 2002/02/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 222 
EP  - 231 
DO  - 10.1101/gr.224202 
VL  - 12 
IS  - 2 
UR  - http://genome.cshlp.org/content/12/2/222.abstract 
N2  - We recently mapped a quantitative trait locus (QTL) with a major effect on milk composition—particularly fat content—to the centromeric end of bovine chromosome 14. We subsequently exploited linkage disequilibrium to refine the map position of this QTL to a 3-cM chromosome interval bounded by microsatellite markers BULGE13and BULGE09. We herein report the positional candidate cloning of this QTL, involving (1) the construction of a BAC contig spanning the corresponding marker interval, (2) the demonstration that a very strong candidate gene, acylCoA:diacylglycerol acyltransferase (DGAT1), maps to that contig, and (3) the identification of a nonconservative K232A substitution in the DGAT1 gene with a major effect on milk fat content and other milk characteristics.[The sequence data described in this paper have been submitted to the GenBank data library under accession number AY065621.] 
ER  -