@article{Grisart01022002,
author = {Grisart, Bernard and Coppieters, Wouter and Farnir, Frédéric and Karim, Latifa and Ford, Christine and Berzi, Paulette and Cambisano, Nadine and Mni, Myriam and Reid, Suzanne and Simon, Patricia and Spelman, Richard and Georges, Michel and Snell, Russell}, 
title = {Positional Candidate Cloning of a QTL in Dairy Cattle: Identification of a Missense Mutation in the Bovine DGAT1 Gene with Major Effect on Milk Yield and Composition},
volume = {12}, 
number = {2}, 
pages = {222-231}, 
year = {2002}, 
doi = {10.1101/gr.224202}, 
abstract ={We recently mapped a quantitative trait locus (QTL) with a major effect on milk composition—particularly fat content—to the centromeric end of bovine chromosome 14. We subsequently exploited linkage disequilibrium to refine the map position of this QTL to a 3-cM chromosome interval bounded by microsatellite markers BULGE13and BULGE09. We herein report the positional candidate cloning of this QTL, involving (1) the construction of a BAC contig spanning the corresponding marker interval, (2) the demonstration that a very strong candidate gene, acylCoA:diacylglycerol acyltransferase (DGAT1), maps to that contig, and (3) the identification of a nonconservative K232A substitution in the DGAT1 gene with a major effect on milk fat content and other milk characteristics.[The sequence data described in this paper have been submitted to the GenBank data library under accession number AY065621.]}, 
URL = {http://genome.cshlp.org/content/12/2/222.abstract}, 
eprint = {http://genome.cshlp.org/content/12/2/222.full.pdf+html}, 
journal = {Genome Research} 
}