TY - JOUR A1 - VanBuren, Vincent A1 - Piao, Yulan A1 - Dudekula, Dawood B. A1 - Qian, Yong A1 - Carter, Mark G. A1 - Martin, Patrick R. A1 - Stagg, Carole A. A1 - Bassey, Uwem C. A1 - Aiba, Kazuhiro A1 - Hamatani, Toshio A1 - Kargul, George J. A1 - Luo, Amber G. A1 - Kelso, Janet A1 - Hide, Winston A1 - Ko, Minoru S.H. T1 - Assembly, Verification, and Initial Annotation of the NIA Mouse 7.4K cDNA Clone Set Y1 - 2002/12/01 JF - Genome Research JO - Genome Research SP - 1999 EP - 2003 DO - 10.1101/gr.633802 VL - 12 IS - 12 UR - http://genome.cshlp.org/content/12/12/1999.abstract N2 - A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.[The sequence data from this study have been submitted to GenBank under accession nos. BQ550036–BQ563104.] ER -