RT Journal
A1 Nuwaysir, Emile F.
A1 Huang, Wei
A1 Albert, Thomas J.
A1 Singh, Jaz
A1 Nuwaysir, Kate
A1 Pitas, Alan
A1 Richmond, Todd
A1 Gorski, Tom
A1 Berg, James P.
A1 Ballin, Jeff
A1 McCormick, Mark
A1 Norton, Jason
A1 Pollock, Tim
A1 Sumwalt, Terry
A1 Butcher, Lawrence
A1 Porter, DeAnn
A1 Molla, Michael
A1 Hall, Christine
A1 Blattner, Fred
A1 Sussman, Michael R.
A1 Wallace, Rodney L.
A1 Cerrina, Franco
A1 Green, Roland D.
T1 Gene Expression Analysis Using Oligonucleotide Arrays Produced by Maskless Photolithography
JF Genome Research 
JO Genome Research 
YR 2002 
FD November 01 
VO 12 
IS 11 
SP 1749 
OP 1755 
DO 10.1101/gr.362402 
UL http://genome.cshlp.org/content/12/11/1749.abstract 
AB Microarrays containing 195,000 in situ synthesized oligonucleotide features have been created using a benchtop, maskless photolithographic instrument. This instrument, the Maskless Array Synthesizer (MAS), uses a digital light processor (DLP) developed by Texas Instruments. The DLP creates the patterns of UV light used in the light-directed synthesis of oligonucleotides. This digital mask eliminates the need for expensive and time-consuming chromium masks. In this report, we describe experiments in which we tested this maskless technology for DNA synthesis on glass surfaces. Parameters examined included deprotection rates, repetitive yields, and oligonucleotide length. Custom gene expression arrays were manufactured and hybridized toDrosophila melanogaster and mouse samples. Quantitative PCR was used to validate the gene expression data from the mouse arrays.[The sequence data from this study have been submitted to GEO under accession nos. GPL208, GSM2409, GSM2410, GSM2411, GSM2412, GSM2413, GSM2414, GSE81, GSE82.]