TY  - JOUR
A1  - Nuwaysir, Emile F.
A1  - Huang, Wei
A1  - Albert, Thomas J.
A1  - Singh, Jaz
A1  - Nuwaysir, Kate
A1  - Pitas, Alan
A1  - Richmond, Todd
A1  - Gorski, Tom
A1  - Berg, James P.
A1  - Ballin, Jeff
A1  - McCormick, Mark
A1  - Norton, Jason
A1  - Pollock, Tim
A1  - Sumwalt, Terry
A1  - Butcher, Lawrence
A1  - Porter, DeAnn
A1  - Molla, Michael
A1  - Hall, Christine
A1  - Blattner, Fred
A1  - Sussman, Michael R.
A1  - Wallace, Rodney L.
A1  - Cerrina, Franco
A1  - Green, Roland D.
T1  - Gene Expression Analysis Using Oligonucleotide Arrays Produced by Maskless Photolithography
Y1  - 2002/11/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1749 
EP  - 1755 
DO  - 10.1101/gr.362402 
VL  - 12 
IS  - 11 
UR  - http://genome.cshlp.org/content/12/11/1749.abstract 
N2  - Microarrays containing 195,000 in situ synthesized oligonucleotide features have been created using a benchtop, maskless photolithographic instrument. This instrument, the Maskless Array Synthesizer (MAS), uses a digital light processor (DLP) developed by Texas Instruments. The DLP creates the patterns of UV light used in the light-directed synthesis of oligonucleotides. This digital mask eliminates the need for expensive and time-consuming chromium masks. In this report, we describe experiments in which we tested this maskless technology for DNA synthesis on glass surfaces. Parameters examined included deprotection rates, repetitive yields, and oligonucleotide length. Custom gene expression arrays were manufactured and hybridized toDrosophila melanogaster and mouse samples. Quantitative PCR was used to validate the gene expression data from the mouse arrays.[The sequence data from this study have been submitted to GEO under accession nos. GPL208, GSM2409, GSM2410, GSM2411, GSM2412, GSM2413, GSM2414, GSE81, GSE82.] 
ER  -