RT Journal
A1 Bhattacharyya, Anamitra
A1 Stilwagen, Stephanie
A1 Reznik, Gary
A1 Feil, Helene
A1 Feil, William S.
A1 Anderson, Iain
A1 Bernal, Axel
A1 D'Souza, Mark
A1 Ivanova, Natalia
A1 Kapatral, Vinayak
A1 Larsen, Niels
A1 Los, Tamara
A1 Lykidis, Athanasios
A1 Selkov, Eugene
A1 Walunas, Theresa L.
A1 Purcell, Alexander
A1 Edwards, Rob A.
A1 Hawkins, Trevor
A1 Haselkorn, Robert
A1 Overbeek, Ross
A1 Kyrpides, Nikos C.
A1 Predki, Paul F.
T1 Draft Sequencing and Comparative Genomics of Xylella fastidiosa Strains Reveal Novel Biological Insights
JF Genome Research 
JO Genome Research 
YR 2002 
FD October 01 
VO 12 
IS 10 
SP 1556 
OP 1563 
DO 10.1101/gr.370702 
UL http://genome.cshlp.org/content/12/10/1556.abstract 
AB Draft sequencing is a rapid and efficient method for determining the near-complete sequence of microbial genomes. Here we report a comparative analysis of one complete and two draft genome sequences of the phytopathogenic bacterium, Xylella fastidiosa, which causes serious disease in plants, including citrus, almond, and oleander. We present highlights of an in silico analysis based on a comparison of reconstructions of core biological subsystems. Cellular pathway reconstructions have been used to identify a small number of genes, which are likely to reside within the draft genomes but are not captured in the draft assembly. These represented only a small fraction of all genes and were predominantly large and small ribosomal subunit protein components. By using this approach, some of the inherent limitations of draft sequence can be significantly reduced. Despite the incomplete nature of the draft genomes, it is possible to identify several phage-related genes, which appear to be absent from the draft genomes and not the result of insufficient sequence sampling. This region may therefore identify potential host-specific functions. Based on this first functional reconstruction of a phytopathogenic microbe, we spotlight an unusual respiration machinery as a potential target for biological control. We also predicted and developed a new defined growth medium forXylella.[The sequence data from this study have been submitted to GenBank under accession nos. NC_002723 (X. fastidiosa Almond [Dixon]) and NC_002722 (X. fastidiosaOleander [Ann-1]).