TY  - JOUR
A1  - Bhattacharyya, Anamitra
A1  - Stilwagen, Stephanie
A1  - Reznik, Gary
A1  - Feil, Helene
A1  - Feil, William S.
A1  - Anderson, Iain
A1  - Bernal, Axel
A1  - D'Souza, Mark
A1  - Ivanova, Natalia
A1  - Kapatral, Vinayak
A1  - Larsen, Niels
A1  - Los, Tamara
A1  - Lykidis, Athanasios
A1  - Selkov, Eugene
A1  - Walunas, Theresa L.
A1  - Purcell, Alexander
A1  - Edwards, Rob A.
A1  - Hawkins, Trevor
A1  - Haselkorn, Robert
A1  - Overbeek, Ross
A1  - Kyrpides, Nikos C.
A1  - Predki, Paul F.
T1  - Draft Sequencing and Comparative Genomics of Xylella fastidiosa Strains Reveal Novel Biological Insights
Y1  - 2002/10/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1556 
EP  - 1563 
DO  - 10.1101/gr.370702 
VL  - 12 
IS  - 10 
UR  - http://genome.cshlp.org/content/12/10/1556.abstract 
N2  - Draft sequencing is a rapid and efficient method for determining the near-complete sequence of microbial genomes. Here we report a comparative analysis of one complete and two draft genome sequences of the phytopathogenic bacterium, Xylella fastidiosa, which causes serious disease in plants, including citrus, almond, and oleander. We present highlights of an in silico analysis based on a comparison of reconstructions of core biological subsystems. Cellular pathway reconstructions have been used to identify a small number of genes, which are likely to reside within the draft genomes but are not captured in the draft assembly. These represented only a small fraction of all genes and were predominantly large and small ribosomal subunit protein components. By using this approach, some of the inherent limitations of draft sequence can be significantly reduced. Despite the incomplete nature of the draft genomes, it is possible to identify several phage-related genes, which appear to be absent from the draft genomes and not the result of insufficient sequence sampling. This region may therefore identify potential host-specific functions. Based on this first functional reconstruction of a phytopathogenic microbe, we spotlight an unusual respiration machinery as a potential target for biological control. We also predicted and developed a new defined growth medium forXylella.[The sequence data from this study have been submitted to GenBank under accession nos. NC_002723 (X. fastidiosa Almond [Dixon]) and NC_002722 (X. fastidiosaOleander [Ann-1]). 
ER  -