RT Journal
A1 Cloix, Catherine
A1 Tutois, Sylvie
A1 Yukawa, Yasushi
A1 Mathieu, Olivier
A1 Cuvillier, Claudine
A1 Espagnol, Marie-Claude
A1 Picard, Georges
A1 Tourmente, Sylvette
T1 Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNA
JF Genome Research 
JO Genome Research 
YR 2002 
FD January 01 
VO 12 
IS 1 
SP 132 
OP 144 
DO 10.1101/gr.181301 
UL http://genome.cshlp.org/content/12/1/132.abstract 
AB One major 5S RNA, 120 bases long, was revealed by an analysis of mature 5S RNA from tissues, developmental stages, and polysomes inArabidopsis thaliana. Minor 5S RNA were also found, varying from the major one by one or two base substitutions; 5S rDNA units from each 5S array of the Arabidopsis genome were isolated by PCR using CIC yeast artificial chromosomes (YACs) mapped on the different loci. By using a comparison of the 5S DNA and RNA sequences, we could show that both major and minor 5S transcripts come from only two of the genomic 5S loci: chromosome 4 and chromosome 5 major block. Other 5S loci are either not transcribed or produce rapidly degraded 5S transcripts. Analysis of the 5′- and 3′-DNA flanking sequence has permitted the definition of specific signatures for each 5S rDNA array.[EMBL accession nos: AF330825-AF331032;AF335777-AF335873.]