RT Journal
A1 Lim, Alex
A1 Dimalanta, Eileen T.
A1 Potamousis, Konstantinos D.
A1 Yen, Galex
A1 Apodoca, Jennifer
A1 Tao, Chunhong
A1 Lin, Jieyi
A1 Qi, Rong
A1 Skiadas, John
A1 Ramanathan, Arvind
A1 Perna, Nicole T.
A1 Plunkett, Guy
A1 Burland, Valerie
A1 Mau, Bob
A1 Hackett, Jeremiah
A1 Blattner, Frederick R.
A1 Anantharaman, Thomas S.
A1 Mishra, Bhubaneswar
A1 Schwartz, David C.
T1 Shotgun Optical Maps of the Whole Escherichia coli O157:H7 Genome
JF Genome Research 
JO Genome Research 
YR 2001 
FD September 01 
VO 11 
IS 9 
SP 1584 
OP 1593 
DO 10.1101/gr.172101 
UL http://genome.cshlp.org/content/11/9/1584.abstract 
AB We have constructed NheI and XhoI optical maps ofEscherichia coli O157:H7 solely from genomic DNA molecules to provide a uniquely valuable scaffold for contig closure and sequence validation. E. coli O157:H7 is a common pathogen found in contaminated food and water. Our approach obviated the need for the analysis of clones, PCR products, and hybridizations, because maps were constructed from ensembles of single DNA molecules. Shotgun sequencing of bacterial genomes remains labor-intensive, despite advances in sequencing technology. This is partly due to manual intervention required during the last stages of finishing. The applicability of optical mapping to this problem was enhanced by advances in machine vision techniques that improved mapping throughput and created a path to full automation of mapping. Comparisons were made between maps and sequence data that characterized sequence gaps and guided nascent assemblies.