TY  - JOUR
A1  - Lim, Alex
A1  - Dimalanta, Eileen T.
A1  - Potamousis, Konstantinos D.
A1  - Yen, Galex
A1  - Apodoca, Jennifer
A1  - Tao, Chunhong
A1  - Lin, Jieyi
A1  - Qi, Rong
A1  - Skiadas, John
A1  - Ramanathan, Arvind
A1  - Perna, Nicole T.
A1  - Plunkett, Guy
A1  - Burland, Valerie
A1  - Mau, Bob
A1  - Hackett, Jeremiah
A1  - Blattner, Frederick R.
A1  - Anantharaman, Thomas S.
A1  - Mishra, Bhubaneswar
A1  - Schwartz, David C.
T1  - Shotgun Optical Maps of the Whole Escherichia coli O157:H7 Genome
Y1  - 2001/09/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1584 
EP  - 1593 
DO  - 10.1101/gr.172101 
VL  - 11 
IS  - 9 
UR  - http://genome.cshlp.org/content/11/9/1584.abstract 
N2  - We have constructed NheI and XhoI optical maps ofEscherichia coli O157:H7 solely from genomic DNA molecules to provide a uniquely valuable scaffold for contig closure and sequence validation. E. coli O157:H7 is a common pathogen found in contaminated food and water. Our approach obviated the need for the analysis of clones, PCR products, and hybridizations, because maps were constructed from ensembles of single DNA molecules. Shotgun sequencing of bacterial genomes remains labor-intensive, despite advances in sequencing technology. This is partly due to manual intervention required during the last stages of finishing. The applicability of optical mapping to this problem was enhanced by advances in machine vision techniques that improved mapping throughput and created a path to full automation of mapping. Comparisons were made between maps and sequence data that characterized sequence gaps and guided nascent assemblies. 
ER  -