@article{Lim01092001,
author = {Lim, Alex and Dimalanta, Eileen T. and Potamousis, Konstantinos D. and Yen, Galex and Apodoca, Jennifer and Tao, Chunhong and Lin, Jieyi and Qi, Rong and Skiadas, John and Ramanathan, Arvind and Perna, Nicole T. and Plunkett, Guy and Burland, Valerie and Mau, Bob and Hackett, Jeremiah and Blattner, Frederick R. and Anantharaman, Thomas S. and Mishra, Bhubaneswar and Schwartz, David C.}, 
title = {Shotgun Optical Maps of the Whole Escherichia coli O157:H7 Genome},
volume = {11}, 
number = {9}, 
pages = {1584-1593}, 
year = {2001}, 
doi = {10.1101/gr.172101}, 
abstract ={We have constructed NheI and XhoI optical maps ofEscherichia coli O157:H7 solely from genomic DNA molecules to provide a uniquely valuable scaffold for contig closure and sequence validation. E. coli O157:H7 is a common pathogen found in contaminated food and water. Our approach obviated the need for the analysis of clones, PCR products, and hybridizations, because maps were constructed from ensembles of single DNA molecules. Shotgun sequencing of bacterial genomes remains labor-intensive, despite advances in sequencing technology. This is partly due to manual intervention required during the last stages of finishing. The applicability of optical mapping to this problem was enhanced by advances in machine vision techniques that improved mapping throughput and created a path to full automation of mapping. Comparisons were made between maps and sequence data that characterized sequence gaps and guided nascent assemblies.}, 
URL = {http://genome.cshlp.org/content/11/9/1584.abstract}, 
eprint = {http://genome.cshlp.org/content/11/9/1584.full.pdf+html}, 
journal = {Genome Research} 
}