TY  - JOUR
A1  - Piao, Yulan
A1  - Ko, Naomi T.
A1  - Lim, Meng K.
A1  - Ko, Minoru S.H.
T1  - Construction of Long-Transcript Enriched cDNA Libraries from Submicrogram Amounts of Total RNAs by a Universal PCR Amplification Method
Y1  - 2001/09/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1553 
EP  - 1558 
DO  - 10.1101/gr.185501 
VL  - 11 
IS  - 9 
UR  - http://genome.cshlp.org/content/11/9/1553.abstract 
N2  - Here we report a novel design of linker primer that allows one to differentially amplify long tracts (average 3.0 kb with size ranges of 1–7 kb) or short DNAs (average 1.5 kb with size ranges of 0.5–3 kb) from a complex mixture. The method allows one to generate cDNA libraries enriched for long transcripts without size selection of insert DNAs. One representative library from newborn kidney includes 70% of clones bearing ATG start codons. A comparable library has been generated from 20 mouse blastocysts, containing only ∼40 ng of total RNA. This universal PCR amplification scheme can provide a route to isolate very large cDNAs, even if they are expressed at very low levels.[The sequence data described in this paper have been submitted to the GenBank data library under accession numbersBG060207–BG062928.] 
ER  -