@article{Dong01082001,
author = {Dong, Shoulian and Wang, Eugene and Hsie, Linda and Cao, Yanxiang and Chen, Xiaogiong and Gingeras, Thomas R.}, 
title = {Flexible Use of High-Density Oligonucleotide Arrays for Single-Nucleotide Polymorphism Discovery and Validation},
volume = {11}, 
number = {8}, 
pages = {1418-1424}, 
year = {2001}, 
doi = {10.1101/gr.171101}, 
abstract ={A method for identifying and validating single nucleotide polymorphisms (SNPs) with high-density oligonucleotide arrays without the need for locus-specific polymerase chain reactions (PCR) is described in this report. Genomic DNAs were divided into subsets with complexity of ∼10 Mb by restriction enzyme digestion and gel-based fragment size resolution, ligated to a common adaptor, and amplified with one primer in a single PCR reaction. As a demonstration of this approach, a total of 124 SNPs were located in 190 kb of genomic sequences distributed across the entire human genome by hybridizing to high-density variant detection arrays (VDA). A set of independent validation experiments was conducted for these SNPs employing bead-based affinity selection followed by hybridization of the affinity-selected SNP-containing fragments to the same VDA that was used to identify the SNPs. A total of 98.7% (74/75) of these SNPs were confirmed using both DNA dideoxynucleotide sequencing and the VDA methodologies. With flexible sample preparation, high-density oligonucleotide arrays can be tailored for even larger scale genome-wide SNP discovery as well as validation.}, 
URL = {http://genome.cshlp.org/content/11/8/1418.abstract}, 
eprint = {http://genome.cshlp.org/content/11/8/1418.full.pdf+html}, 
journal = {Genome Research} 
}