RT Journal
A1 Corbeil, Jacques
A1 Sheeter, Dennis
A1 Genini, Davide
A1 Rought, Steffney
A1 Leoni, Lorenzo
A1 Du, Pinyi
A1 Ferguson, Mark
A1 Masys, Daniel R.
A1 Welsh, John B.
A1 Fink, J. Lynn
A1 Sasik, Roman
A1 Huang, David
A1 Drenkow, Jorg
A1 Richman, Douglas D.
A1 Gingeras, Thomas
T1 Temporal Gene Regulation During HIV-1 Infection of Human CD4+ T Cells
JF Genome Research 
JO Genome Research 
YR 2001 
FD July 01 
VO 11 
IS 7 
SP 1198 
OP 1204 
DO 10.1101/gr.180201 
UL http://genome.cshlp.org/content/11/7/1198.abstract 
AB CD4+ T-cell depletion is a characteristic of human immunodeficiency virus type 1 (HIV-1) infection. In this study, modulation of mRNA expression of 6800 genes was monitored simultaneously at eight time points in a CD4+ T-cell line (CEM-GFP) during HIV infection. The responses to infection included: (1) >30% decrease at 72 h after infection in overall host-cell production of monitored mRNA synthesis, with the replacement of host-cell mRNA by viral mRNA, (2) suppression of the expression of selected mitochondrial and DNA repair gene transcripts, (3) increased expression of the proapoptotic gene and its gene p53-induced product Bax, and (4) activation of caspases 2, 3, and 9. The intense HIV-1 transcription resulted in the repression of much cellular RNA expression and was associated with the induction of apoptosis of infected cells but not bystander cells. This choreographed host gene response indicated that the subversion of the cell transcriptional machinery for the purpose of HIV-1 replication is akin to genotoxic stress and represents a major factor leading to HIV-induced apoptosis.