RT Journal
A1 Mayer, Klaus
A1 Murphy, George
A1 Tarchini, Renato
A1 Wambutt, Rolf
A1 Volckaert, Guido
A1 Pohl, Thomas
A1 Düsterhöft, Andreas
A1 Stiekema, Willem
A1 Entian, Karl-Dieter
A1 Terryn, Nancy
A1 Lemcke, Kai
A1 Haase, Dirk
A1 Hall, Caroline R.
A1 van Dodeweerd, Anne-Marie
A1 Tingey, Scott V.
A1 Mewes, Hans-Werner
A1 Bevan, Michael W.
A1 Bancroft, Ian
T1 Conservation of Microstructure between a Sequenced Region of the Genome of Rice and Multiple Segments of the Genome of Arabidopsis thaliana

JF Genome Research 
JO Genome Research 
YR 2001 
FD July 01 
VO 11 
IS 7 
SP 1167 
OP 1174 
DO 10.1101/gr.161701 
UL http://genome.cshlp.org/content/11/7/1167.abstract 
AB The nucleotide sequence was determined for a 340-kb segment of rice chromosome 2, revealing 56 putative protein-coding genes. This represents a density of one gene per 6.1 kb, which is higher than was reported for a previously sequenced segment of the rice genome. Sixteen of the putative genes were supported by matches to ESTs. The predicted products of 29 of the putative genes showed similarity to known proteins, and a further 17 genes showed similarity only to predicted or hypothetical proteins identified in genome sequence data. The region contains a few transposable elements: one retrotransposon, and one transposon. The segment of the rice genome studied had previously been identified as representing a part of rice chromosome 2 that may be homologous to a segment of Arabidopsis chromosome 4. We confirmed the conservation of gene content and order between the two genome segments. In addition, we identified a further four segments of the Arabidopsis genome that contain conserved gene content and order. In total, 22 of the 56 genes identified in the rice genome segment were represented in this set of Arabidopsis genome segments, with at least five genes present, in conserved order, in each segment. These data are consistent with the hypothesis that theArabidopsis genome has undergone multiple duplication events. Our results demonstrate that conservation of the genome microstructure can be identified even between monocot and dicot species. However, the frequent occurrence of duplication, and subsequent microstructure divergence, within plant genomes may necessitate the integration of subsets of genes present in multiple redundant segments to deduce evolutionary relationships and identify orthologous genes.