RT Journal
A1 Dean, Frank B.
A1 Nelson, John R.
A1 Giesler, Theresa L.
A1 Lasken, Roger S.
T1 Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification
JF Genome Research 
JO Genome Research 
YR 2001 
FD June 01 
VO 11 
IS 6 
SP 1095 
OP 1099 
DO 10.1101/gr.180501 
UL http://genome.cshlp.org/content/11/6/1095.abstract 
AB We describe a simple method of using rolling circle amplification to amplify vector DNA such as M13 or plasmid DNA from single colonies or plaques. Using random primers and φ29 DNA polymerase, circular DNA templates can be amplified 10,000-fold in a few hours. This procedure removes the need for lengthy growth periods and traditional DNA isolation methods. Reaction products can be used directly for DNA sequencing after phosphatase treatment to inactivate unincorporated nucleotides. Amplified products can also be used for in vitro cloning, library construction, and other molecular biology applications.