TY  - JOUR
A1  - Dean, Frank B.
A1  - Nelson, John R.
A1  - Giesler, Theresa L.
A1  - Lasken, Roger S.
T1  - Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification
Y1  - 2001/06/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1095 
EP  - 1099 
DO  - 10.1101/gr.180501 
VL  - 11 
IS  - 6 
UR  - http://genome.cshlp.org/content/11/6/1095.abstract 
N2  - We describe a simple method of using rolling circle amplification to amplify vector DNA such as M13 or plasmid DNA from single colonies or plaques. Using random primers and φ29 DNA polymerase, circular DNA templates can be amplified 10,000-fold in a few hours. This procedure removes the need for lengthy growth periods and traditional DNA isolation methods. Reaction products can be used directly for DNA sequencing after phosphatase treatment to inactivate unincorporated nucleotides. Amplified products can also be used for in vitro cloning, library construction, and other molecular biology applications. 
ER  -