RT Journal
A1 Benos, Panayiotis V.
A1 Gatt, Melanie K.
A1 Murphy, Lee
A1 Harris, David
A1 Barrell, Bart
A1 Ferraz, Concepcion
A1 Vidal, Sophie
A1 Brun, Christine
A1 Demaille, Jacques
A1 Cadieu, Edouard
A1 Dreano, Stephane
A1 Gloux, Stéphanie
A1 Lelaure, Valerie
A1 Mottier, Stephanie
A1 Galibert, Francis
A1 Borkova, Dana
A1 Miñana, Belen
A1 Kafatos, Fotis C.
A1 Bolshakov, Slava
A1 Sidén-Kiamos, Inga
A1 Papagiannakis, George
A1 Spanos, Lefteris
A1 Louis, Christos
A1 Madueño, Encarnación
A1 de Pablos, Beatriz
A1 Modolell, Juan
A1 Peter, Annette
A1 Schöttler, Petra
A1 Werner, Meike
A1 Mourkioti, Fotini
A1 Beinert, Nicole
A1 Dowe, Gordon
A1 Schäfer, Ulrich
A1 Jäckle, Herbert
A1 Bucheton, Alain
A1 Callister, Debbie
A1 Campbell, Lorna
A1 Henderson, Nadine S.
A1 McMillan, Paul J.
A1 Salles, Cathy
A1 Tait, Evelyn
A1 Valenti, Phillipe
A1 Saunders, Robert D.C.
A1 Billaud, Alain
A1 Pachter, Lior
A1 Glover, David M.
A1 Ashburner, Michael
T1 From First Base: The Sequence of the Tip of the X Chromosome of Drosophila melanogaster, a Comparison of Two Sequencing Strategies
JF Genome Research 
JO Genome Research 
YR 2001 
FD May 01 
VO 11 
IS 5 
SP 710 
OP 730 
DO 10.1101/gr.173801 
UL http://genome.cshlp.org/content/11/5/710.abstract 
AB We present the sequence of a contiguous 2.63 Mb of DNA extending from the tip of the X chromosome ofDrosophila melanogaster. Within this sequence, we predict 277 protein coding genes, of which 94 had been sequenced already in the course of studying the biology of their gene products, and examples of 12 different transposable elements. We show that an interval between bands 3A2 and 3C2, believed in the 1970s to show a correlation between the number of bands on the polytene chromosomes and the 20 genes identified by conventional genetics, is predicted to contain 45 genes from its DNA sequence. We have determined the insertion sites ofP-elements from 111 mutant lines, about half of which are in a position likely to affect the expression of novel predicted genes, thus representing a resource for subsequent functional genomic analysis. We compare the European Drosophila Genome Project sequence with the corresponding part of the independently assembled and annotated Joint Sequence determined through “shotgun” sequencing. Discounting differences in the distribution of known transposable elements between the strains sequenced in the two projects, we detected three major sequence differences, two of which are probably explained by errors in assembly; the origin of the third major difference is unclear. In addition there are eight sequence gaps within the Joint Sequence. At least six of these eight gaps are likely to be sites of transposable elements; the other two are complex. Of the 275 genes in common to both projects, 60% are identical within 1% of their predicted amino-acid sequence and 31% show minor differences such as in choice of translation initiation or termination codons; the remaining 9% show major differences in interpretation.[All of the sequences analyzed in this paper have been deposited in the EMBL-Bank database under the following accession nos.: AL009146,AL009147, AL009171, AL009188–AL009196, AL021067, AL021086,AL021106–AL021108, AL021726, AL021728, AL022017, AL022018, AL022139,AL023873, AL023874, AL023893, AL024453, AL024455–AL024457, AL024485,AL030993, AL030994, AL031024–AL031028, AL031128, AL031173, AL031366,AL031367, AL031581–AL031583, AL031640, AL031765, AL031883, AL031884,AL034388, AL034544, AL035104, AL035105, AL035207, AL035245, AL035331,AL035632, AL049535, AL050231, AL050232, AL109630, AL121804, AL121806,AL132651, AL132792, AL132797, AL133503–AL133506, AL138678, AL138971,AL138972, and Z98269. A single file (FASTA format) of the 2.6-Mb contig is available fromftp://ftp.ebi.ac.uk/pub/databases/edgp/contigs/contig_1.fa.]