TY  - JOUR
A1  - Benos, Panayiotis V.
A1  - Gatt, Melanie K.
A1  - Murphy, Lee
A1  - Harris, David
A1  - Barrell, Bart
A1  - Ferraz, Concepcion
A1  - Vidal, Sophie
A1  - Brun, Christine
A1  - Demaille, Jacques
A1  - Cadieu, Edouard
A1  - Dreano, Stephane
A1  - Gloux, Stéphanie
A1  - Lelaure, Valerie
A1  - Mottier, Stephanie
A1  - Galibert, Francis
A1  - Borkova, Dana
A1  - Miñana, Belen
A1  - Kafatos, Fotis C.
A1  - Bolshakov, Slava
A1  - Sidén-Kiamos, Inga
A1  - Papagiannakis, George
A1  - Spanos, Lefteris
A1  - Louis, Christos
A1  - Madueño, Encarnación
A1  - de Pablos, Beatriz
A1  - Modolell, Juan
A1  - Peter, Annette
A1  - Schöttler, Petra
A1  - Werner, Meike
A1  - Mourkioti, Fotini
A1  - Beinert, Nicole
A1  - Dowe, Gordon
A1  - Schäfer, Ulrich
A1  - Jäckle, Herbert
A1  - Bucheton, Alain
A1  - Callister, Debbie
A1  - Campbell, Lorna
A1  - Henderson, Nadine S.
A1  - McMillan, Paul J.
A1  - Salles, Cathy
A1  - Tait, Evelyn
A1  - Valenti, Phillipe
A1  - Saunders, Robert D.C.
A1  - Billaud, Alain
A1  - Pachter, Lior
A1  - Glover, David M.
A1  - Ashburner, Michael
T1  - From First Base: The Sequence of the Tip of the X Chromosome of Drosophila melanogaster, a Comparison of Two Sequencing Strategies
Y1  - 2001/05/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 710 
EP  - 730 
DO  - 10.1101/gr.173801 
VL  - 11 
IS  - 5 
UR  - http://genome.cshlp.org/content/11/5/710.abstract 
N2  - We present the sequence of a contiguous 2.63 Mb of DNA extending from the tip of the X chromosome ofDrosophila melanogaster. Within this sequence, we predict 277 protein coding genes, of which 94 had been sequenced already in the course of studying the biology of their gene products, and examples of 12 different transposable elements. We show that an interval between bands 3A2 and 3C2, believed in the 1970s to show a correlation between the number of bands on the polytene chromosomes and the 20 genes identified by conventional genetics, is predicted to contain 45 genes from its DNA sequence. We have determined the insertion sites ofP-elements from 111 mutant lines, about half of which are in a position likely to affect the expression of novel predicted genes, thus representing a resource for subsequent functional genomic analysis. We compare the European Drosophila Genome Project sequence with the corresponding part of the independently assembled and annotated Joint Sequence determined through “shotgun” sequencing. Discounting differences in the distribution of known transposable elements between the strains sequenced in the two projects, we detected three major sequence differences, two of which are probably explained by errors in assembly; the origin of the third major difference is unclear. In addition there are eight sequence gaps within the Joint Sequence. At least six of these eight gaps are likely to be sites of transposable elements; the other two are complex. Of the 275 genes in common to both projects, 60% are identical within 1% of their predicted amino-acid sequence and 31% show minor differences such as in choice of translation initiation or termination codons; the remaining 9% show major differences in interpretation.[All of the sequences analyzed in this paper have been deposited in the EMBL-Bank database under the following accession nos.: AL009146,AL009147, AL009171, AL009188–AL009196, AL021067, AL021086,AL021106–AL021108, AL021726, AL021728, AL022017, AL022018, AL022139,AL023873, AL023874, AL023893, AL024453, AL024455–AL024457, AL024485,AL030993, AL030994, AL031024–AL031028, AL031128, AL031173, AL031366,AL031367, AL031581–AL031583, AL031640, AL031765, AL031883, AL031884,AL034388, AL034544, AL035104, AL035105, AL035207, AL035245, AL035331,AL035632, AL049535, AL050231, AL050232, AL109630, AL121804, AL121806,AL132651, AL132792, AL132797, AL133503–AL133506, AL138678, AL138971,AL138972, and Z98269. A single file (FASTA format) of the 2.6-Mb contig is available fromftp://ftp.ebi.ac.uk/pub/databases/edgp/contigs/contig_1.fa.] 
ER  -