RT Journal
A1 Scheetz, Todd E.
A1 Raymond, Michael R.
A1 Nishimura, Darryl Y.
A1 McClain, Ann
A1 Roberts, Chad
A1 Birkett, Clay
A1 Gardiner, Jack
A1 Zhang, Jie
A1 Butters, Nichole
A1 Sun, Christine
A1 Kwitek-Black, Anne
A1 Jacob, Howard
A1 Casavant, Thomas L.
A1 Soares, M. Bento
A1 Sheffield, Val C.
T1 Generation of a High-Density Rat EST Map
JF Genome Research 
JO Genome Research 
YR 2001 
FD March 01 
VO 11 
IS 3 
SP 497 
OP 502 
DO 10.1101/gr.151601 
UL http://genome.cshlp.org/content/11/3/497.abstract 
AB We have developed a high-density EST map of the rat, consisting of >11,000 ESTs. These ESTs were placed on a radiation hybrid framework map of genetic markers spanning all 20 rat autosomes, plus the X chromosome. The framework maps have a total size of ∼12,400 cR, giving an average correspondence of 240 kb/cR. The frameworks are all LOD 3 chromosomal maps consisting of 775 radiation-hybrid-mapped genetic markers and ESTs. To date, we have generated radiation-hybrid-mapping data for >14,000 novel ESTs identified by our Rat Gene Discovery and Mapping Project (http://ratEST.uiowa.edu), from which we have placed >11,000 on our framework maps. To minimize mapping errors, ESTs were mapped in duplicate and consensus RH vectors produced for use in the placement procedure. This EST map was then used to construct high-density comparative maps between rat and human and rat and mouse. These maps will be a useful resource for positional cloning of genes for rat models of human diseases and in the creation and verification of a tiling set of map order for the upcoming rat-genome sequencing.