RT Journal
A1 Osoegawa, Kazutoyo
A1 Mammoser, Aaron G.
A1 Wu, Chenyan
A1 Frengen, Eirik
A1 Zeng, Changjiang
A1 Catanese, Joseph J.
A1 de Jong, Pieter J.
T1 A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome
JF Genome Research 
JO Genome Research 
YR 2001 
FD March 01 
VO 11 
IS 3 
SP 483 
OP 496 
DO 10.1101/gr.169601 
UL http://genome.cshlp.org/content/11/3/483.abstract 
AB A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large average insert size (178 kb) has been constructed to provide the intermediate substrate for the international genome sequencing effort. The DNA was obtained from a single anonymous volunteer, whose identity was protected through a double-blind donor selection protocol. DNA fragments were generated by partial digestion with EcoRI (library segments 1–4: 24-fold) and MboI (segment 5: sixfold) and cloned into the pBACe3.6 and pTARBAC1 vectors, respectively. The quality of the library was assessed by extensive analysis of 169 clones for rearrangements and artifacts. Eighteen BACs (11%) revealed minor insert rearrangements, and none was chimeric. This BAC library, designated as “RPCI-11,” has been used widely as the central resource for insert-end sequencing, clone fingerprinting, high-throughput sequence analysis and as a source of mapped clones for diagnostic and functional studies.The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AQ936150–AQ936491.]