@article{Osoegawa01032001,
author = {Osoegawa, Kazutoyo and Mammoser, Aaron G. and Wu, Chenyan and Frengen, Eirik and Zeng, Changjiang and Catanese, Joseph J. and de Jong, Pieter J.}, 
title = {A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome},
volume = {11}, 
number = {3}, 
pages = {483-496}, 
year = {2001}, 
doi = {10.1101/gr.169601}, 
abstract ={A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large average insert size (178 kb) has been constructed to provide the intermediate substrate for the international genome sequencing effort. The DNA was obtained from a single anonymous volunteer, whose identity was protected through a double-blind donor selection protocol. DNA fragments were generated by partial digestion with EcoRI (library segments 1–4: 24-fold) and MboI (segment 5: sixfold) and cloned into the pBACe3.6 and pTARBAC1 vectors, respectively. The quality of the library was assessed by extensive analysis of 169 clones for rearrangements and artifacts. Eighteen BACs (11%) revealed minor insert rearrangements, and none was chimeric. This BAC library, designated as “RPCI-11,” has been used widely as the central resource for insert-end sequencing, clone fingerprinting, high-throughput sequence analysis and as a source of mapped clones for diagnostic and functional studies.The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AQ936150–AQ936491.]}, 
URL = {http://genome.cshlp.org/content/11/3/483.abstract}, 
eprint = {http://genome.cshlp.org/content/11/3/483.full.pdf+html}, 
journal = {Genome Research} 
}