RT Journal
A1 Konno, Hideaki
A1 Fukunishi, Yoshifumi
A1 Shibata, Kazuhiro
A1 Itoh, Masayoshi
A1 Carninci, Piero
A1 Sugahara, Yuichi
A1 Hayashizaki, Yoshihide
T1 Computer-Based Methods for the Mouse Full-Length cDNA Encyclopedia: Real-Time Sequence Clustering for Construction of a Nonredundant cDNA Library
JF Genome Research 
JO Genome Research 
YR 2001 
FD February 01 
VO 11 
IS 2 
SP 281 
OP 289 
DO 10.1101/gr.145701 
UL http://genome.cshlp.org/content/11/2/281.abstract 
AB We developed computer-based methods for constructing a nonredundant mouse full-length cDNA library. Our cDNA library construction process comprises assessment of library quality, sequencing the 3′ ends of inserts and clustering, and completing a re-array to generate a nonredundant library from a redundant one. After the cDNA libraries are generated, we sequence the 5′ ends of the inserts to check the quality of the library; then we determine the sequencing priority of each library. Selected libraries undergo large-scale sequencing of the 3′ ends of the inserts and clustering of the tag sequences. After clustering, the nonredundant library is constructed from the original libraries, which have redundant clones. All libraries, plates, clones, sequences, and clusters are uniquely identified, and all information is saved in the database according to this identifier. At press time, our system has been in place for the past two years; we have clustered 939,725 3′ end sequences into 127,385 groups from 227 cDNA libraries/sublibraries (seehttp://genome.gse.riken.go.jp/).[The sequence data described in this paper have been submitted to the DDBJ data library under accession nos. AV00011–AV175734, AV204013–AV382295, andBB561685–BB609425.]