TY  - JOUR
A1  - Konno, Hideaki
A1  - Fukunishi, Yoshifumi
A1  - Shibata, Kazuhiro
A1  - Itoh, Masayoshi
A1  - Carninci, Piero
A1  - Sugahara, Yuichi
A1  - Hayashizaki, Yoshihide
T1  - Computer-Based Methods for the Mouse Full-Length cDNA Encyclopedia: Real-Time Sequence Clustering for Construction of a Nonredundant cDNA Library
Y1  - 2001/02/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 281 
EP  - 289 
DO  - 10.1101/gr.145701 
VL  - 11 
IS  - 2 
UR  - http://genome.cshlp.org/content/11/2/281.abstract 
N2  - We developed computer-based methods for constructing a nonredundant mouse full-length cDNA library. Our cDNA library construction process comprises assessment of library quality, sequencing the 3′ ends of inserts and clustering, and completing a re-array to generate a nonredundant library from a redundant one. After the cDNA libraries are generated, we sequence the 5′ ends of the inserts to check the quality of the library; then we determine the sequencing priority of each library. Selected libraries undergo large-scale sequencing of the 3′ ends of the inserts and clustering of the tag sequences. After clustering, the nonredundant library is constructed from the original libraries, which have redundant clones. All libraries, plates, clones, sequences, and clusters are uniquely identified, and all information is saved in the database according to this identifier. At press time, our system has been in place for the past two years; we have clustered 939,725 3′ end sequences into 127,385 groups from 227 cDNA libraries/sublibraries (seehttp://genome.gse.riken.go.jp/).[The sequence data described in this paper have been submitted to the DDBJ data library under accession nos. AV00011–AV175734, AV204013–AV382295, andBB561685–BB609425.] 
ER  -