RT Journal
A1 Schalkwyk, Leonard C.
A1 Cusack, Brian
A1 Dunkel, Ilona
A1 Hopp, Martina
A1 Kramer, Markus
A1 Palczewski, Stefanie
A1 Piefke, Jutta
A1 Scheel, Sabine
A1 Weiher, Michael
A1 Wenske, Gunther
A1 Lehrach, Hans
A1 Himmelbauer, Heinz
T1 Advanced Integrated Mouse YAC Map Including BAC Framework
JF Genome Research 
JO Genome Research 
YR 2001 
FD December 01 
VO 11 
IS 12 
SP 2142 
OP 2150 
DO 10.1101/gr.176201 
UL http://genome.cshlp.org/content/11/12/2142.abstract 
AB Functional characterization of the mouse genome requires the availability of a comprehensive physical map to obtain molecular access to chromosomal regions of interest. Positional cloning remains a crucial way of linking phenotype with particular genes. A key step and frequent stumbling block in positional cloning is making a contig of a genetically defined candidate region. The most efficient first step is isolating YAC (Yeast Artificial Chromosome) clones. A robust, detailed YAC contig map is thus an important tool. Employing Interspersed Repetitive Sequence (IRS)-PCR genomics, we have generated an advanced second-generation YAC contig map of the mouse genome that doubles both the depth of clones and the density of markers available. In addition to the primarily YAC-based map, we located 1942 BAC (Bacterial Artificial Chromosome) clones. This allows us to present for the first time a dense framework of BACs spanning the genome of the mouse, which, for instance, can serve as a nucleus for genomic sequencing. Four large-insert mouse YAC libraries from three different strains are included in our data, and our analysis incorporates the data of Hunter et al. and Nusbaum et al. There is a total of 20,205 markers on the final map, 12,033 from our own data, and a total of 56,093 YACs, of which 44,401 are positive for more than one marker.[The sequence data described in this paper have been submitted to the GenBank data library under accession nos. BH174059–BH175013.]