RT Journal
A1 Quintero, Marı́a José
A1 Montesinos, Marı́a Luz
A1 Herrero, Antonia
A1 Flores, Enrique
T1 Identification of Genes Encoding Amino Acid Permeases by Inactivation of Selected ORFs from the Synechocystis Genomic Sequence
JF Genome Research 
JO Genome Research 
YR 2001 
FD December 01 
VO 11 
IS 12 
SP 2034 
OP 2040 
DO 10.1101/gr.196301 
UL http://genome.cshlp.org/content/11/12/2034.abstract 
AB Genes encoding elements of four amino acid permeases were identified by insertional inactivation of ORFs from the genomic sequence of the cyanobacterium Synechocystis sp. strain PCC 6803 whose putative products are homologous to amino acid permease proteins from other bacteria. A transport system for neutral amino acids and histidine and a transport system for basic amino acids and glutamine were identified as ABC-type transporters, whereas Na+-dependent transport of glutamate was found to be mediated by at least two systems, the secondary permease GltS and a TRAP-type transporter. Except for GltS, substrate specificities of the identified permeases do not match those of previously characterized systems homologous to these permeases.