RT Journal
A1 Kudoh, Tetsuhiro
A1 Tsang, Michael
A1 Hukriede, Neil A.
A1 Chen, Xiongfong
A1 Dedekian, Michael
A1 Clarke, Christopher J.
A1 Kiang, Anne
A1 Schultz, Stephanie
A1 Epstein, Jonathan A.
A1 Toyama, Reiko
A1 Dawid, Igor B.
T1 A Gene Expression Screen in Zebrafish Embryogenesis
JF Genome Research 
JO Genome Research 
YR 2001 
FD December 01 
VO 11 
IS 12 
SP 1979 
OP 1987 
DO 10.1101/gr.209601 
UL http://genome.cshlp.org/content/11/12/1979.abstract 
AB A screen for developmentally regulated genes was conducted in the zebrafish, a system offering substantial advantages for the study of the molecular genetics of vertebrate embryogenesis. Clones from a normalized cDNA library from early somitogenesis stages were picked randomly and tested by high-throughput in situ hybridization for restricted expression in at least one of four stages of development. Among 2765 clones that were screened, a total of 347 genes with patterns judged to be restricted were selected. These clones were subjected to partial sequence analysis, allowing recognition of functional motifs in 163 among them. In addition, a portion of the clones were mapped with the aid of the LN54 radiation hybrid panel. The usefulness of the in situ hybridization screening approach is illustrated by describing several new markers for the characteristic structure in the fish embryo named the yolk syncytial layer, and for different regions of the developing brain.