TY  - JOUR
A1  - Sano, Yuri
A1  - Shimada, Tokihiko
A1  - Nakashima, Hiroshi
A1  - Nicholson, Rhonda H.
A1  - Eliason, James F.
A1  - Kocarek, Thomas A.
A1  - Ko, Minoru S.H.
T1  - Random Monoallelic Expression of Three Genes Clustered within 60 kb of Mouse t Complex Genomic DNA
Y1  - 2001/11/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1833 
EP  - 1841 
DO  - 10.1101/gr.194301 
VL  - 11 
IS  - 11 
UR  - http://genome.cshlp.org/content/11/11/1833.abstract 
N2  - Mammals achieve gene dosage control by (1) random X-chromosome inactivation in females, (2) parental origin-specific imprinting of selected autosomal genes, and (3) random autosomal inactivation. Genes belonging to the third category of epigenetic phenomenon are just now emerging, with only six identified so far. Here we report three additional genes, Nubp2, Igfals, and Jsap1, that show 50%-methylated CpG sites by Southern blot analyses and primarily monoallelic expression in single-cell allele-specific RT-PCR analysis of bone marrow stromal cells and hepatocytes. Furthermore, we show that, in contrast to X inactivation, alleles can switch between active and inactive states during the formation of daughter cells. These three genes are the first in their category to exist as a tight cluster, in the proximal region of mouse chromosome 17, providing a thus far unique example of a region of autosomal random monoallelic expression. 
ER  -