RT Journal
A1 Zhao, Shaying
A1 Shatsman, Sofiya
A1 Ayodeji, Bola
A1 Geer, Keita
A1 Tsegaye, Getahun
A1 Krol, Margaret
A1 Gebregeorgis, Elizabeth
A1 Shvartsbeyn, Alla
A1 Russell, Daniel
A1 Overton, Larry
A1 Jiang, Lingxia
A1 Dimitrov, George
A1 Tran, Kevin
A1 Shetty, Jyoti
A1 Malek, Joel A.
A1 Feldblyum, Tamara
A1 Nierman, William C.
A1 Fraser, Claire M.
T1 Mouse BAC Ends Quality Assessment and Sequence Analyses
JF Genome Research 
JO Genome Research 
YR 2001 
FD October 01 
VO 11 
IS 10 
SP 1736 
OP 1745 
DO 10.1101/gr.179201 
UL http://genome.cshlp.org/content/11/10/1736.abstract 
AB A large-scale BAC end-sequencing project at The Institute for Genomic Research (TIGR) has generated one of the most extensive sets of sequence markers for the mouse genome to date. With a sequencing success rate of >80%, an average read length of 485 bp, and ABI3700 capillary sequencers, we have generated 449,234 nonredundant mouse BAC end sequences (mBESs) with 218 Mb total from 257,318 clones from libraries RPCI-23 and RPCI-24, representing 15× clone coverage, 7% sequence coverage, and a marker every 7 kb across the genome. A total of 191,916 BACs have sequences from both ends providing 12× genome coverage. The average Q20 length is 406 bp and 84% of the bases havephred quality scores ≥ 20. RPCI-24 mBESs have more Q20 bases and longer reads on average than RPCI-23 sequences. ABI3700 sequencers and the sample tracking system ensure that > 95% of mBESs are associated with the right clone identifiers. We have found that a significant fraction of mBESs contains L1 repeats and ∼48% of the clones have both ends with ≥ 100 bp contiguous unique Q20 bases. About 3% mBESs match ESTs and > 70% of matches were conserved between the mouse and the human or the rat. Approximately 0.1% mBESs contain STSs. About 0.2% mBESs match human finished sequences and > 70% of these sequences have EST hits. The analyses indicate that our high-quality mouse BAC end sequences will be a valuable resource to the community.