RT Journal
A1 Myakishev, Maxim V.
A1 Khripin, Yuri
A1 Hu, Stella
A1 Hamer, Dean H.
T1 High-Throughput SNP Genotyping by Allele-Specific PCR with Universal Energy-Transfer-Labeled Primers
JF Genome Research 
JO Genome Research 
YR 2001 
FD January 01 
VO 11 
IS 1 
SP 163 
OP 169 
DO 10.1101/gr.157901 
UL http://genome.cshlp.org/content/11/1/163.abstract 
AB We have developed a new method for high-throughput genotyping of single nucleotide polymorphisms (SNPs). The technique involves PCR amplification of genomic DNA with two tailed allele-specific primers that introduce priming sites for universal energy-transfer-labeled primers. The output of red and green light is conveniently scored using a fluorescence plate reader. The new method, which was validated on nine model SNPs, is well suited for high-throughput, automated genotyping because it requires only one reaction per SNP, it is performed in a single tube with no post-PCR handling, the same energy-transfer-labeled primers are used for all analyses, and the instrumentation is inexpensive. Possible applications include multiple-candidate gene analysis, genomewide scans, and medical diagnostics.