TY  - JOUR
A1  - Myakishev, Maxim V.
A1  - Khripin, Yuri
A1  - Hu, Stella
A1  - Hamer, Dean H.
T1  - High-Throughput SNP Genotyping by Allele-Specific PCR with Universal Energy-Transfer-Labeled Primers
Y1  - 2001/01/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 163 
EP  - 169 
DO  - 10.1101/gr.157901 
VL  - 11 
IS  - 1 
UR  - http://genome.cshlp.org/content/11/1/163.abstract 
N2  - We have developed a new method for high-throughput genotyping of single nucleotide polymorphisms (SNPs). The technique involves PCR amplification of genomic DNA with two tailed allele-specific primers that introduce priming sites for universal energy-transfer-labeled primers. The output of red and green light is conveniently scored using a fluorescence plate reader. The new method, which was validated on nine model SNPs, is well suited for high-throughput, automated genotyping because it requires only one reaction per SNP, it is performed in a single tube with no post-PCR handling, the same energy-transfer-labeled primers are used for all analyses, and the instrumentation is inexpensive. Possible applications include multiple-candidate gene analysis, genomewide scans, and medical diagnostics. 
ER  -