@article{Myakishev01012001,
author = {Myakishev, Maxim V. and Khripin, Yuri and Hu, Stella and Hamer, Dean H.}, 
title = {High-Throughput SNP Genotyping by Allele-Specific PCR with Universal Energy-Transfer-Labeled Primers},
volume = {11}, 
number = {1}, 
pages = {163-169}, 
year = {2001}, 
doi = {10.1101/gr.157901}, 
abstract ={We have developed a new method for high-throughput genotyping of single nucleotide polymorphisms (SNPs). The technique involves PCR amplification of genomic DNA with two tailed allele-specific primers that introduce priming sites for universal energy-transfer-labeled primers. The output of red and green light is conveniently scored using a fluorescence plate reader. The new method, which was validated on nine model SNPs, is well suited for high-throughput, automated genotyping because it requires only one reaction per SNP, it is performed in a single tube with no post-PCR handling, the same energy-transfer-labeled primers are used for all analyses, and the instrumentation is inexpensive. Possible applications include multiple-candidate gene analysis, genomewide scans, and medical diagnostics.}, 
URL = {http://genome.cshlp.org/content/11/1/163.abstract}, 
eprint = {http://genome.cshlp.org/content/11/1/163.full.pdf+html}, 
journal = {Genome Research} 
}