RT Journal
A1 Band, Mark R.
A1 Larson, Joshua H.
A1 Rebeiz, Mark
A1 Green, Cheryl A.
A1 Heyen, D. Wayne
A1 Donovan, Jena
A1 Windish, Ryan
A1 Steining, Chad
A1 Mahyuddin, Prapti
A1 Womack, James E.
A1 Lewin, Harris A.
T1 An Ordered Comparative Map of the Cattle and Human Genomes
JF Genome Research 
JO Genome Research 
YR 2000 
FD September 01 
VO 10 
IS 9 
SP 1359 
OP 1368 
DO 10.1101/gr.145900 
UL http://genome.cshlp.org/content/10/9/1359.abstract 
AB A cattle–human whole-genome comparative map was constructed using parallel radiation hybrid (RH) mapping in conjunction with EST sequencing, database mining for unmapped cattle genes, and a predictive bioinformatics approach (COMPASS) for targeting specific homologous regions. A total of 768 genes were placed on the RH map in addition to 319 microsatellites used as anchor markers. Of these, 638 had human orthologs with mapping data, thus permitting construction of an ordered comparative map. The large number of ordered loci revealed ⋝ 105 conserved segments between the two genomes. The comparative map suggests that 41 translocation events, a minimum of 54 internal rearrangements, and repositioning of all but one centromere can account for the observed organizations of the cattle and human genomes. In addition, the COMPASS in silico mapping tool was shown to be 95% accurate in its ability to predict cattle chromosome location from random sequence data, demonstrating this tool to be valuable for efficient targeting of specific regions for detailed mapping. The comparative map generated will be a cornerstone for elucidating mammalian chromosome phylogeny and the identification of genes of agricultural importance.“Ought we, for instance, to begin by discussing each separate species—in virtue of some common element of their nature, and proceed from this as a basis for the consideration of them separately?” from Aristotle, On the Parts of Animals, 350 B.C.E.[The sequence data described in this paper have been submitted to the GenBank data library under accession nos.AW244888-AW244897, AW261132-AW261195, AW266849-AW267161,AW289175-AW289430, AW428566-AW428607, AW621146, AW621147.]