RT Journal
A1 Eickhoff, Holger
A1 Schuchhardt, Johannes
A1 Ivanov, Igor
A1 Meier-Ewert, Sebastian
A1 O'Brien, John
A1 Malik, Arif
A1 Tandon, Neeraj
A1 Wolski, Eryk-Witold
A1 Rohlfs, Elke
A1 Nyarsik, Lajos
A1 Reinhardt, Richard
A1 Nietfeld, Wilfried
A1 Lehrach, Hans
T1 Tissue Gene Expression Analysis Using Arrayed Normalized cDNA Libraries
JF Genome Research 
JO Genome Research 
YR 2000 
FD August 01 
VO 10 
IS 8 
SP 1230 
OP 1240 
DO 10.1101/gr.10.8.1230 
UL http://genome.cshlp.org/content/10/8/1230.abstract 
AB We have used oligonucleotide-fingerprinting data on 60,000 cDNA clones from two different mouse embryonic stages to establish a normalized cDNA clone set. The normalized set of 5,376 clones represents different clusters and therefore, in almost all cases, different genes. The inserts of the cDNA clones were amplified by PCR and spotted on glass slides. The resulting arrays were hybridized with mRNA probes prepared from six different adult mouse tissues. Expression profiles were analyzed by hierarchical clustering techniques. We have chosen radioactive detection because it combines robustness with sensitivity and allows the comparison of multiple normalized experiments. Sensitive detection combined with highly effective clustering algorithms allowed the identification of tissue-specific expression profiles and the detection of genes specifically expressed in the tissues investigated. The obtained results are publicly available (http://www.rzpd.de) and can be used by other researchers as a digital expression reference.[The sequence data described in this paper have been submitted to the EMBL data library under accession nos. AL360374–AL36537.]