TY  - JOUR
A1  - Barry, Alyssa E.
A1  - Bateman, Melissa
A1  - Howman, Emily V.
A1  - Cancilla, Michael R.
A1  - Tainton, Kellie M.
A1  - Irvine, Danielle V.
A1  - Saffery, Richard
A1  - Choo, K.H. Andy
T1  - The 10q25 Neocentromere and its Inactive Progenitor Have Identical Primary Nucleotide Sequence: Further Evidence for Epigenetic Modification
Y1  - 2000/06/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 832 
EP  - 838 
DO  - 10.1101/gr.10.6.832 
VL  - 10 
IS  - 6 
UR  - http://genome.cshlp.org/content/10/6/832.abstract 
N2  - We have previously localized the core centromere protein-binding domain of a 10q25.2-derived neocentromere to an 80-kb genomic region. Detailed analysis has indicated that the 80-kb neocentromere (NC) DNA has a similar overall organization to the corresponding region on a normal chromosome 10 (HC) DNA, derived from a genetically unrelated CEPH individual. Here we report sequencing of the HC DNA and its comparison to the NC sequence. Single-base differences were observed at a maximum rate of 4.6 per kb; however, no deletions, insertions, or other structural rearrangements were detected. To investigate whether the observed changes, or subsets of these, might be de novo mutations involved in neocentromerization (i.e., in committing a region of a chromosome to neocentromere formation), the progenitor DNA (PnC) from which the NC DNA descended, was cloned and sequenced. Direct comparison of the PnC and NC sequences revealed 100% identity, suggesting that the differences between NC and HC DNA are single nucleotide polymorphisms (SNPs) and that formation of the 10q25.2 NC did not involve a change in DNA sequence in the core centromere protein-binding NC region. This is the first study in which a cloned NC DNA has been compared directly with its inactive progenitor DNA at the primary sequence level. The results form the basis for future sequence comparison outside the core protein-binding domain, and provide direct support for the involvement of an epigenetic mechanism in neocentromerization.[The sequences in this paper have been submitted to GenBank under accession nos. AF222855 (not yet available) for HC; AF042484 for NCI; AF222854 (not yet available) for NCII; andAF222856 (not yet available) for PnC.] 
ER  -