RT Journal
A1 Murphy, William J.
A1 Sun, Shan
A1 Chen, Zhang-qun
A1 Yuhki, Naoya
A1 Hirschmann, Deborah
A1 Menotti-Raymond, Marilyn
A1 O'Brien, Stephen J.
T1 A Radiation Hybrid Map of the Cat Genome: Implications for Comparative Mapping
JF Genome Research 
JO Genome Research 
YR 2000 
FD May 01 
VO 10 
IS 5 
SP 691 
OP 702 
DO 10.1101/gr.10.5.691 
UL http://genome.cshlp.org/content/10/5/691.abstract 
AB Ordered gene maps of mammalian species are becoming increasingly valued in assigning gene variants to function in human and animal models, as well as recapitulating the natural history of genome organization. To extend this power to the domestic cat, a radiation hybrid (RH) map of the cat was constructed integrating 424 Type I-coding genes with 176 microsatellite markers, providing coverage over all 20 feline chromosomes. Alignment of parallel RH maps of human and cat reveal 100 conserved segments ordered (CSOs) between the species, nearly three times the number observed with reciprocal chromosome painting analyses. The observed number is equivalent to theoretical predictions of the number of conserved segments to be found between cat and human, implying that 300–400 Type I gene markers is sufficient to reveal nearly all conserved segments for species that exhibit the most frequently observed “slow” rate of genome reorganization. The cat–human RH map comparisons provide a new genomic tool for comparative gene mapping in the cat and related Felidae, and provide confirmation that the cat genome organization is remarkably conserved compared with human. These data demonstrate that ordered RH-based gene maps provide the most precise assessment of comparing genomes, short of contig construction or full-sequence determination.[The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AZ081249–AZ081287 andAW646734–AW646956.]