TY  - JOUR
A1  - Henikoff, Jorja G.
A1  - Henikoff, Steven
T1  - 
Drosophila Genomic Sequence Annotation Using the BLOCKS+ Database
Y1  - 2000/04/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 543 
EP  - 546 
DO  - 10.1101/gr.10.4.543 
VL  - 10 
IS  - 4 
UR  - http://genome.cshlp.org/content/10/4/543.abstract 
N2  - A simple and general homology-based method for gene finding was applied to the 2.9-Mb Drosophila melanogaster Adh region, the target sequence of the Genome Annotation Assessment Project (GASP). Each strand of the entire sequence was used as query of theBLOCKS+ database of conserved regions of proteins. This led to functional assignments for more than one-third of the genes and two-thirds of the transposons. Considering the enormous size of the query, the fact that only two false-positive matches were reported emphasizes the high selectivity of protein family-based methods for gene finding. We used the search results to improveBLOCKS+ by identifying compositionally biased blocks. Our results confirm that protein family databases can be used effectively in automated sequence annotation efforts. 
ER  -