@article{Henikoff01042000,
author = {Henikoff, Jorja G. and Henikoff, Steven}, 
title = {
Drosophila Genomic Sequence Annotation Using the BLOCKS+ Database},
volume = {10}, 
number = {4}, 
pages = {543-546}, 
year = {2000}, 
doi = {10.1101/gr.10.4.543}, 
abstract ={A simple and general homology-based method for gene finding was applied to the 2.9-Mb Drosophila melanogaster Adh region, the target sequence of the Genome Annotation Assessment Project (GASP). Each strand of the entire sequence was used as query of theBLOCKS+ database of conserved regions of proteins. This led to functional assignments for more than one-third of the genes and two-thirds of the transposons. Considering the enormous size of the query, the fact that only two false-positive matches were reported emphasizes the high selectivity of protein family-based methods for gene finding. We used the search results to improveBLOCKS+ by identifying compositionally biased blocks. Our results confirm that protein family databases can be used effectively in automated sequence annotation efforts.}, 
URL = {http://genome.cshlp.org/content/10/4/543.abstract}, 
eprint = {http://genome.cshlp.org/content/10/4/543.full.pdf+html}, 
journal = {Genome Research} 
}